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Abstract

Mixed Infection in Microsporidial Keratitis In A Rural Tertiary Care Hospital

Aim

To study the prevalence of Mixed infection in Microsporidial keratitis infection from a rural tertiary care hospital.

Method

Corneal scraping samples from October 2018 to October 2019 had come to Microbiology central laboratory at Narayana nethralaya hospital,Bommasandra Bangalore. 94 samples were collected using a surgical scalpel blade no. 15 and it was subjected to microscopic Gram stain examination, 10%Pottasium hydroxide (KOH), calcoflour mount and culture techniques. 16 samples showed microsporidia in KOH and Calcoflour mount, 3 samples showed bacteria in Gram stain along with microsporidia out of which, 1 Gram negative bacilli and 2 Gram positive cocci. Gram positive cocci showed growth on blood and chocolate agar after 48 hours of incubation at 37ºC, Gram negative bacilli did not showed any growth. Gram positive cocci was identified as Staphylococcus epidermidis by catalase and coagulase biochemical tests.

Results

Out of 94 samples, 16 samples showed microsporidia in KOH and Calcoflour mount, 3 samples showed bacteria in Gram stain along with microsporidia out of which, 1 Gram negative bacilli and 2 Gram positive cocci. Gram positive cocci showed growth on blood and chocolate agar after 48 hours of incubation at 37ºC, Gram negative bacilli did not showed any growth. Gram positive cocci was identified as Staphylococcus epidermidis by catalase and coagulase biochemical tests.

Conclusion

Out of 16 microsporidial cases three samples were mixed with Gram positive and Gram negative bacteria, Gram negative mixed infection was seen in above 50 years age group and Gram positive mixed infection was seen in 30-45 years age group.


Author(s):

Shilpa K1, Venkataramana A1, Nandini C1



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