In Burkina Faso, the effectiveness of the various disinfectants against Aspergillus spores used on medical devices has not yet been demonstrated. Thus the effectiveness of glutaraldehyde, one of the high-level disinfectants, used most often in the disinfection of these medical devices, deserves to be proven through a scientific study. Therefore, our study aimed to determine the minimum inhibitory concentration as a function of contact time of glutaraldehyde on Aspergillus flavus, Aspergillus niger and Aspergillus fumigatus.